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Fig. 4.

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Fig. 4. Structural analysis of group C and D by electron tomography. (A) Electron micrographs (upper) and rendering of synapses (down) of 21 days neuron cells from brain-derived neurotrophic factor (BDNF) treatment (Group C), Latrunculin A treatment after BDNF pre-incubation (Group D). Average of the number of synaptic vesicles/presynaptic density (active zone) length and total number of synaptic vesicle were calculated and compared with the group C. (B) Average of proximal synaptic vesicle and tethered vesicles per synapse. (C) Average of number of connector. Color list as follows; synapse (red), presynaptic density (PSD, blue), synaptic vesicle (SV, yellow), and actin filament (sea green). **p<0.01, ***p<0.001, vs. group C. Arrows; synaptic vesicle filament. Scale bars=200 nm.
Applied Microscopy 2018;48:55-61
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