Fig. 3. Immunolabelling was performed with a primary antibody: Chemicon MAB922 & secondary antibody: Gold-conjugated (15 nm) protein A (supplied by Prof. Su Jin Kim), and then refinement & osmication and routine processing for scanning electron microscope (SEM), freeze drying and observed by field emission SEM (S-4700; Hitachi). The white spots in the picture are 15 nm gold. In the transmission electron microscope, the gold particles appear black because they interfere with the transmission of electrons ().

Applied Microscopy 2018;48:87-95

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