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Role of Actin Filament on Synaptic Vesicle Pooling in Cultured Hippocampal Neuron
Applied Microscopy 2018;48:55-61
Published online September 30, 2018
© 2018 Korean Society of Microscopy.

Se Jeong Lee1,†, Hyun-Wook Kim1,†, Ji Eun Na1, DaSom Kim1, Dai Hyun Kim1, Jae Ryun Ryu1, Woong Sun1, Im Joo Rhyu1,2,*

1Department of Anatomy, Korea University College of Medicine, Seoul 02841, Korea
2Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, Seoul 02841, Korea
Correspondence to: Rhyu IJ, http://orcid.org/0000-0002-5558-6278 Tel: +82-2-2286-1149 Fax: +82-2-929-5696 E-mail: irhyu@korea.ac.kr
These authors contributed equally to this work.
Received September 22, 2018; Revised September 30, 2018; Accepted September 30, 2018.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The synaptic vesicle is a specialized structure in presynaptic terminals that stores various neurotransmitters. The actin filament has been proposed for playing an important role in mobilizing synaptic vesicles. To understand the role of actin filament on synaptic vesicle pooling, we characterized synaptic vesicles and actin filament after treatment of brain-derived neurotrophic factor (BDNF) or Latrunculin A on primary cultured neuron from rat embryo hippocampus. Western blots revealed that BDNF treatment increased the expression of synapsin I protein, but Latrunculin A treatment decreased the synapsin I protein expression. The increased expression of synapsin I after BDNF disappeared by the treatment of Latrunculin A. Three-dimensional (3D) tomography of synapse showed that more synaptic vesicles localized near the active zone and total number of synaptic vesicles increased after treatment of BDNF. But the number of synaptic vesicle was 2.5-fold reduced in presynaptic terminals and the loss of filamentous network was observed after Latrunculin A application. The treatment of Latruculin A after preincubation of BDNF group showed that synaptic vesicle number was similar to that of control group, but filamentous structures were not restored. These data suggest that the actin filament plays a significant role in synaptic vesicles pooling in presynaptic terminals.
Keywords : Synaptic vesicle, Actin filament, Brain-derived neurotrophic factor, Latrunculin A, Electron tomography


September 2018, 48 (3)
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