Chang-Hyun Park*, Hong Lim Kim1, Byung-Joon Chang2, Sang Hoon Lee3, Byung Soo Chang4, Chun-Sik Bae5, Ik-Hyun Cho6, Dong Heui Kim7, Jung-Mi Han2, Ji Eun Na8, Byung-Jin Choi, Sang-Sik Kim, Hyun-Wook Kim, Jee-Woong Kim9, Im Joo Rhyu8, and Chang-Sub Uhm8
Medical Science Research Center, Korea University College of Medicine, Seoul 02841, Korea, 1Integrative Research Support Center, The Catholic University of Korea, Seoul 06591, Korea, 2College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea, 3Korea Brain Research Institute, Daegu 41062, Korea, 4Department of Cosmetology, Hanseo University, Seosan 31962, Korea, 5College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea, 6Department of Convergence Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea, 7Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju 26426, Korea, 8Department of Anatomy, Korea University College of Medicine, Seoul 02841, Korea, 9Division of Biosafety Evaluation and Control, Korea National Institute of Health, Cheongju 28159, Korea
Correspondence to: Park CH, http://orcid.org/0000-0002-7306-035X, Tel: +82-2-2286-1323, Fax: +82-2-2286-1329, E-mail: kmemno1@empal.com
Received July 24, 2018; Revised November 5, 2018; Accepted November 11, 2018.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Abstract
Immunoelectron microscopy using an antigen-antibody reaction in an electron microscope is a very useful tool to identify the components of a tissue in an electron microscope. Many researchers also use immunoelectron microscopy. Nonetheless, immunoelectron microscopy is rarely introduced systematically, and immunoelectron microscopy can be carried out without fully understanding the principles, and cases of poor understanding can often be seen in the vicinity. Therefore, in order to make it easier to understand, we will first introduce the principles of immunoelectron microscopy and describe practical methods.
- Keywords : Pre-embedding, Post-embedding, Immuno cytochemistry, Correlative light and electron microscopy
- References
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- Melo, RC, Morgan, E, Monahan-Earley, R, Dvorak, AM, and Weller, PF (2014). Pre-embedding immunogold labeling to optimize protein localization at subcellular compartiments and membrane microdomains of leukocytes. Nat Protoc. 9, 2382-2394.
- December 2018, 48 (4)
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Author ORCID Information-
Chang-Hyun Park
https://orcid.org/0000-0002-7306-035X
